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This educational video from the DNA Learning Center provides a detailed walkthrough of the library preparation process for Nanopore 16s sequencing, specifically focusing on PCR setup and the addition of labeled indexes. Learn the complete workflow through step-by-step demonstrations including introduction (00:00), preparing reactions for cleaning (03:46), pooling samples (06:46), magnetic bead cleanup (10:10), washing beads (14:12), sample elution (18:41), and adding rapid adapters (22:38). Designed specifically for educators implementing Nanopore technology in classroom settings and course-based undergraduate research experiences (CUREs), this 27-minute guide is part of a comprehensive series explaining Nanopore sequencing fundamentals through demonstration experiments. Access the full protocol at nanopore4edu.org for additional resources to facilitate classroom implementation.
