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This 12-minute tutorial from the DNA Learning Center provides a step-by-step guide on PCR setup for Nanopore DNA sequencing, specifically focusing on 16s sequencing with labeled indexes. Learn about the entire process through three key segments: an introduction to the technique, detailed PCR setup instructions, and the procedure for adding barcoding primers/indices. Designed specifically for educators implementing Nanopore technology in classroom settings, particularly for course-based undergraduate research experiences (CUREs), this video is part of a comprehensive series explaining Nanopore sequencing fundamentals through demonstration experiments. Access the complete protocol for 16s sequencing on the nanopore4edu.org website to incorporate this cutting-edge DNA sequencing technology into your teaching curriculum.
