Educator's Guide to Nanopore 16S: DNA Extraction

This tutorial demonstrates column-based DNA purification using a Norgen microbiome kit as part of the Educator's Guide to Nanopore 16s sequencing. Follow along with the detailed extraction process, starting with an introduction to the procedure, then progressing through sample lysis, transfer and addition of binding buffers, loading samples onto columns, washing the columns, and finally drying columns and elution. Designed specifically for educators looking to incorporate Nanopore DNA sequencing into classroom settings, particularly for course-based undergraduate research experiences (CUREs), this 43-minute video is part of a series explaining Nanopore sequencing fundamentals through demonstration experiments. Access the complete protocol on the nanopore4edu.org website for additional guidance on implementing this technique in educational environments.