Educator's Guide to Nanopore Microbial Isolates: DNA Isolation II - Binding and Elution
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This educational video continues the NEB Monarch protocol for Nanopore DNA sequencing, focusing on binding DNA to capture beads and eluting the final sample. Follow along with the detailed demonstration of the precipitation process (00:00), bead retention techniques (10:24), and final elution procedures (15:00). Part of an educator-focused series designed to make Nanopore sequencing accessible for classroom use, particularly for course-based undergraduate research experiences (CUREs). The 24-minute guide provides practical demonstrations and resources to help educators implement DNA sequencing in their teaching. Additional resources available at nanopore4edu.org.
Syllabus
00:00 Continuing on to precipitation
10:24 Bead retention
15:00 Final elution
Taught by
DNA Learning Center