DNA Learning Center Courses

Educator's Guide to Nanopore - Microbial Isolates: DNA Isolation I (Lysis and Precipitation)

Discover the process of isolating bacterial DNA using the NEB Monarch kit, including lysis, needle sheering, homogenization, and protein separation techniques for Nanopore sequencing.

• YouTube
• 46 minutes
• On-Demand
• Free Video

Educator's Guide to Nanopore - Microbial Isolates: Preparing and Loading Flow Cell

Discover the step-by-step process of preparing and loading a MinION flow cell for Nanopore DNA sequencing, with detailed instructions for educators implementing this technology in classroom settings.

• YouTube
• 22 minutes
• On-Demand
• Free Video

Educator's Guide to Nanopore Microbial Isolates: Library Prep - Pool and Clean

Learn the native barcoding protocol for Nanopore DNA sequencing, including pooling samples, attaching adapters, cleaning steps, and final quantification techniques for microbial isolates.

• YouTube
• 33 minutes
• On-Demand
• Free Video

Educator's Guide to Nanopore Microbial Isolates: DNA Isolation II - Binding and Elution

Dive into the NEB Monarch protocol for DNA isolation, learning how to bind DNA on capture beads and elute the final sample for Nanopore sequencing in educational settings.

• YouTube
• 24 minutes
• On-Demand
• Free Video

Educator's Guide to Nanopore Microbial Isolates: Library Prep - DNA End Repair and Barcoding

Discover the process of preparing microbial isolates for Nanopore DNA sequencing, focusing on end repair and barcoding techniques for educational laboratory settings.

• YouTube
• 44 minutes
• On-Demand
• Free Video

Educator's Guide to Nanopore Microbial Isolates: Flow Cell Check, Run, and Output Review

Discover how to use MinKNOW software for Nanopore DNA sequencing, from checking flow cells to monitoring experiments and reviewing outputs, with a focus on classroom implementation.

• YouTube
• 33 minutes
• On-Demand
• Free Video

Educator's Guide to Nanopore - Microbial Isolates: DNA Quantification with Qubit

Learn how to quantify DNA extraction using the Qubit fluorometer, from overview to sample checking, in this educator-focused guide to Nanopore DNA sequencing for classroom use.

• YouTube
• 15 minutes
• On-Demand
• Free Video

Educator's Guide to Nanopore 16S: DNA Extraction

Master the column-based DNA purification process using a Norgen microbiome kit, from sample lysis to elution, as part of the Nanopore sequencing educational series for classroom implementation.

• YouTube
• 43 minutes
• On-Demand
• Free Video

Educator's Guide to Nanopore 16S: Library Preparation

Dive into the library preparation process for Nanopore 16s sequencing, covering PCR setup, index labeling, sample pooling, magnetic bead cleanup, and adapter addition for classroom implementation.

• YouTube
• 27 minutes
• On-Demand
• Free Video

Educator's Guide to Nanopore 16S: Flongle Flow Cell Loading

Learn the step-by-step process of preparing and loading a Flongle flow cell for Nanopore DNA sequencing, from preparing solutions to adding buffers and libraries, designed for educators implementing this technology in classroom settings.

• YouTube
• 13 minutes
• On-Demand
• Free Video

Educator's Guide to Nanopore 16S Sequencing: PCR Setup and Barcoding

Discover the process of PCR setup and barcoding primers for Nanopore DNA sequencing, designed specifically for educators implementing this technology in classroom settings.

• YouTube
• 12 minutes
• On-Demand
• Free Video

Educator's Guide to Nanopore 16S: Managing Your Device and Reagents

Learn how to manage your Nanopore DNA sequencer, including unboxing, handling flow cells, and storing reagents for effective use in educational settings, especially for undergraduate research experiences.

• YouTube
• 16 minutes
• On-Demand
• Free Video