Educator's Guide to Nanopore - Microbial Isolates: DNA Isolation I (Lysis and Precipitation)

This 46-minute tutorial from DNA Learning Center demonstrates the first steps of DNA isolation from microbial isolates for Nanopore sequencing, focusing on lysis and precipitation techniques. Follow along as instructors use an NEB Monarch kit to isolate bacteria, perform lysis procedures, and prepare samples for precipitation. The video breaks down the process into clear segments: starting with an overview of the protocol, then covering specific techniques for processing Gram-negative bacteria, needle sheering and homogenization methods, and finally the application of RNAse A and Protein Separation Solution. Designed specifically for educators implementing Nanopore technology in classroom settings, particularly for course-based undergraduate research experiences (CUREs), this tutorial is part of a comprehensive series explaining Nanopore sequencing fundamentals through demonstration experiments. Additional resources are available at nanopore4edu.org.