Educator's Guide to Nanopore - Microbial Isolates: Preparing and Loading Flow Cell

This 22-minute tutorial from DNA Learning Center demonstrates the final steps of preparing a microbial isolate library and loading a MinION flow cell for Nanopore DNA sequencing. Follow along with detailed instructions for sample dilution and preparation in the first segment, then learn how to prepare the priming mix and load the library onto the flow cell. Designed specifically for educators implementing Nanopore technology in classroom settings, particularly for course-based undergraduate research experiences (CUREs), this video is part of a comprehensive series explaining Nanopore sequencing fundamentals through demonstration experiments. The content is organized in two main sections: sample dilution and preparation (00:00-06:08) and preparation of priming mix, library, and loading (06:09-end). For additional educational resources on implementing Nanopore sequencing in teaching environments, visit nanopore4edu.org.