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This 44-minute tutorial from the DNA Learning Center demonstrates the library preparation process for Nanopore sequencing of microbial isolates, focusing on DNA end repair and barcoding techniques. Learn how to use a native barcoding kit to prepare isolates for sequencing, with detailed explanations of the protocol overview and end-preparation process (00:00-37:01), followed by instructions for adding barcode adapters (37:02-44:00). Designed as part of an educator-focused introduction to Nanopore DNA sequencing, this video provides valuable resources and approaches to implement Nanopore technology in classroom settings, particularly for course-based undergraduate research experiences (CUREs). Explore the fundamentals of Nanopore sequencing through demonstration experiments and find additional resources at nanopore4edu.org.
