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This tutorial continues the native barcoding protocol for Nanopore DNA sequencing, covering essential steps including pooling barcoded samples, attaching final adapters, and performing all necessary cleaning procedures. Learn the complete process through detailed demonstrations of stopping barcode ligation, pooling and cleaning samples (0:00), adding sequencing adapters (10:48), performing sequencing adapter clean-up (15:52), and conducting final quantification (23:36). Part of an educator-focused series designed to make Nanopore technology more accessible for classroom use, particularly for course-based undergraduate research experiences (CUREs). The 33-minute video provides practical guidance for implementing this DNA sequencing technology in educational settings. Additional resources available at nanopore4edu.org.
