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Learn metagenomics analysis using Kraken 2 in OmicsBox. Covers data preprocessing, taxonomic classification, visualization, and differential abundance analysis for shotgun sequencing data.
Detailed tutorial on variant calling from WGS data using GATK best practices, covering quality control, alignment, duplicate marking, base recalibration, and variant discovery with HaplotypeCaller.
Comprehensive guide to VCF and gVCF file formats in bioinformatics, covering structure, interpretation, and key concepts like genotypes, alleles, and variant recording for genomic analysis.
Comprehensive guide to DESeq2 for differential gene expression analysis in RNA-seq data, covering statistical models, normalization methods, and key steps in the analysis pipeline.
Learn to annotate cell types in single-cell RNA-Seq data using SingleR. Explore annotation strategies, reference datasets, and diagnostic tools for accurate cell type assignment and visualization.
Explore somatic variant calling with Mutect2, covering key concepts, challenges, and hands-on demonstration of variant identification, filtering, and annotation using GATK best practices.
Explore advanced cell-annotation techniques for single-cell RNA-Seq data using SingleR, including multiple reference datasets, visualization strategies, and harmonized labeling approaches.
Comprehensive guide to motif discovery and transcription factor binding site analysis, covering basics, tools, and practical demonstrations using Homer software for ChIP-seq data analysis.
Understand why NGS samples generate multiple FASTQ files through library prep, flowcells, lane multiplexing, and learn proper data organization strategies for bioinformatics workflows.
Comprehensive guide to Conda and virtual environments, covering installation, package management, and environment creation for Python and R projects. Includes practical demonstrations and comparisons between different tools.
Comprehensive guide for downstream analysis of single-cell ATAC-Seq data using R and Signac. Covers integration with RNA-Seq, differential accessibility analysis, and visualization of genomic regions.
Detailed tutorial on filtering and annotating genetic variants using GATK tools. Covers hard filtering, genotype refinement, and Funcotator for comprehensive variant analysis in bioinformatics workflows.
Learn step-by-step Weighted Gene Co-expression Network Analysis (WGCNA) for RNA-Seq data, covering data manipulation, outlier detection, normalization, module identification, and visualization techniques.
Step-by-step tutorial on single-cell trajectory analysis using Monocle3 and Seurat. Covers theory, workflow, and practical implementation in R, including data processing, visualization, and gene expression analysis along trajectories.
Step-by-step tutorial on processing RNA-Seq data: quality control, trimming, alignment, and quantification. Covers tools like FastQC, Trimmomatic, HISAT2, and featureCounts, with comparisons of aligners and best practices.
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