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XuetangX

Microbiology

Shenyang Agricultural University via XuetangX

Overview

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The significance of the relationship between microorganisms and humankind cannot be overstated.
As indispensable members of the human living environment, microorganisms drive the cycling of essential elements such as carbon and nitrogen within ecosystems and serve as the fundamental nutritional base of ecological food chains and webs. Yet, the diseases and disasters caused by microorganisms are equally critical and demand attention. Faced with these “foes and friends” of humanity, how can we transform potential threats into beneficial allies?

In this course, we will step into the world of microorganisms to study their morphology, physiology, ecology, major groups, and vital life processes. We will explore the intricate mechanisms governing microbial growth, reproduction, and metabolic regulation, while mastering essential laboratory techniques such as microscopic observation, staining, aseptic procedures, isolation and cultivation, and microbial monitoring.

Through the study of this course, you will not only appreciate the astonishing diversity and beauty of the microbial world but also gain insight into the pivotal roles and emerging developments of microorganisms in industrial and agricultural production, as well as in daily human life. Ultimately, you will acquire the ability to analyze and address scientific and practical problems related to microbiology.




Syllabus

  • Introduction
    • Microorganisms and Their Characteristics
    • Brief History of the Development of Microbiology
  • Prokaryotic Microorganisms
    • Bacterial Morphology and Size
    • General Structure of Bacteria (Cell Wall)
    • General Structure of Bacteria (Gram Staining)
    • General Structure of Bacteria (Cell Membrane, Cytoplasm, and Nucleoid Region)
    • Specialized Structures of Bacteria
    • Bacterial Reproduction and Colony Characteristics
    • Actinomycetes
  • Eukaryotic Microorganisms
    • Fungal Morphology
    • Fungal Reproduction
    • Fungal Classification and Representative Genera
  • Microbial Nutrition and Energy Metabolism
    • Microbial Nutrition
    • Types of Nutritional Modes
    • Mechanisms of Nutrient Uptake
    • Culture Media
    • Microbial Metabolism
  • Microbial Growth and Regulation
    • Microbial Growth and Measurement Methods
    • Typical Bacterial Growth Curve
    • Factors Affecting Microbial Growth — Temperature and Oxygen
    • Factors Affecting Microbial Growth — pH, Water Activity, and Osmotic Pressure
    • Control of Harmful Microorganisms — Physical Methods
    • Control of Harmful Microorganisms — Chemical Methods
  • Viruses
    • Fundamental Characteristics of Viruses
    • Viral Structure and Classification
    • Viruses of Prokaryotic Microorganisms — Bacteriophages
    • Viruses of Eukaryotic Organisms
    • Subviral Agents
  • Microbial Ecology
    • Interactions Between Microorganisms and the Environment
    • Microorganisms and Biogeochemical Cycles in Nature
  • Microbial Genetics and Variation
    • Material Basis of Genetic Variation
    • Genetic Recombination in Bacteria
    • Gene Mutation and Mutagenic Breeding
  • Advances in Microbiological Research
    • Emerging Technologies and New Developments in Microbiology — Molecular Biology Techniques
    • Microorganisms and Sustainable Human Development
  • Final Examination
    • Laboratory Experiments
      • Microbiology Laboratory Setup and Requirements
      • Unique Experimental Techniques in Microbiology
      • Use of the Oil-Immersion Microscope (Theoretical Explanation)
      • Use of the Oil-Immersion Microscope (Operational Demonstration)
      • Simple Bacterial Staining Techniques (Theoretical Explanation)
      • Simple Bacterial Staining Techniques (Operational Demonstration)
      • Gram Staining Techniques (Theoretical Explanation)
      • Gram Staining Techniques (Operational Demonstration)
      • Observation of Actinomycete Morphology (Theoretical Explanation)
      • Observation of Actinomycete Morphology (Operational Demonstration)
      • Observation of Fungal Morphology (Theoretical Explanation)
      • Observation of Yeast Morphology (Operational Demonstration)
      • Observation of Rhizopus Morphology (Operational Demonstration)
      • Preparation and Sterilization of Culture Media (Theoretical Explanation)
      • Preparation and Sterilization of Culture Media (Operational Demonstration)
      • Isolation of Pure Microbial Cultures (Theoretical Explanation)
      • Isolation of Pure Microbial Cultures (Operational Demonstration)
      • Observation of Various Microbial Morphologies (Theoretical Explanation)
      • Observation of Various Microbial Morphologies (Operational Demonstration)
      • Detection of Coliform Bacteria in Water Samples (Theoretical Explanation)
      • Bacteria in Water Samples (Operational Demonstration)

    Taught by

    Liu Lingzhi, Li Bingxue, Ding Rui, Zhang Zhiyong, Niu Xuguang, Song Lichao, and Xiao Yinong

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