Mass Spectrometry and its application in Molecular Medicine

Week 1:
L-1: Fundamentals of soft ionization mass spectrometry, Electrospray ionization mass spectrometry (ESI-MS), Matrix Assisted Laser Desorption Ionization mass spectrometry (MALDI-MS).L2: Interpretation of mass spectra: Monoisotopic mass, Average mass, Isotopic distribution of peaks in the mass spectra, Introduction to Molecular Medicine.L3: Deconvolution of mass spectra, Resolution and Mass accuracy. Introduction of LC/ESI-MS platform with examples.L4: Application of LC/ESI-MS in the quantification of glutathionyl hemoglobin, a marker of oxidative stress, in the blood samples.L5: Application of LC/ESI-MS in the quantification of glycated hemoglobin, a marker of Diabetes Mellitus.
Week 2:
L6: Chemical modification of peptides monitored through mass spectra.L7: Collision Induced Dissociation, Tandem mass spectra.L8: De novo sequencing of peptides: Principles and Methods.L9: Example: De novo sequencing from Tandem mass spectra of a peptide.L10: Continued…..Example: De novo sequencing from Tandem mass spectra of a peptide.
Week 3:
L11: Continued…..Example: De novo sequencing from Tandem mass spectra of a peptide.L12: Introduction to proteomics using nanoLC/ESI-MS based bottom-up proteomics approach.L13: Hemoglobinopathies and thalassemia: Identification and characterization using electrophoresis and HPLC based method and its shortcomings.L14: Identification and characterization of hemoglobinopathies using nanoLC/ESI-MS based bottom-up proteomics approach.L15: Continued……Identification and characterization of hemoglobinopathies using nanoLC/ESI-MS based bottom-up proteomics approach.
Week 4:
L16: Example of a genetic variant of hemoglobin that provide false elevation of glycated hemoglobin, a diagnostic biomarker of diabetes mellitus.L17: Functional assay of human hemoglobin using oxygen dissociation curve; Functionally abnormal hemoglobin variants.L18: Iron Deficiency Anemia(IDA); Attempts to combat IDA using iron salts as a food fortificants.L19: Nano ferric pyrophosphate as food fortificant: Synthesis, bioavailability and toxicity assessment in a rat model.L20: Continued……..Nano ferric pyrophosphate as food fortificant: Synthesis, bioavailability and toxicity assessment in a rat model.
Week 5:
L21: Application of nanoLC/ESI-MS platform to search for a biomarker of nano particle induced toxicity in rat plasma using proteomics based approach.L22:Quantitative proteomics: Relative quantification.L23: Quantitative proteomics: Absolute quantification.L24: Gel based proteomics: 2D SDS-PAGE to fractionate proteins in a biological sample.L25: Analysis of 2D gel spots, spot picking, In-gel digestion and elution of proteolytic peptides from gel spots.
Week 6:
L26: Application of MALDI-MS to identify and characterize proteins in the gel spots of a clinical sample isolated from a patient with attempted suicide.L27: Validation of gel-based proteomics biomarker in patients with attempted suicide using nanoLC/ESI-MS based approach and quantitative proteomics.L28: Application of MALDI-MS in the detection of microorganism in a clinical sample.L29: Native mass spectrometry using nano-ESI source, Collisional cooling, Interpretation of native mass spectra with an example of human hemoglobin tetramers.L30: Application of native mass spectrometry in the analysis of an array of glycated hemoglobin in a patient with poorly controlled diabetes mellitus.
Week 7:
L31: Application of native mass spectrometry to calculate dissociation equilibrium constant of a biological complex with an example of quaternary structure of human hemoglobin.L32: Structure-function correlation of human hemoglobin upon oxygenation.L33: Posttranslational modification of human hemoglobin and functional abnormality associated with the modification.L34: Analysis of benzoquinone adduct of hemoglobin from cigarette smoke and environment pollutants using native-MS, its impact on the integrity of tetramer and subsequently on human diseases.L35: Effect of benzoquinones on the architecture and biophysical properties of Red Blood Cells in human blood.
Week 8:

L36: Analysis of genetic variants of human hemoglobin using native-MS with examples of sickle cell hemoglobin (HbS), fetal hemoglobin (HbF), Hb-Beckman.L37: Ion mobility mass spectrometry and its application in the analysis of functionally active conformation of proteins.L38: Assessing cross sectional area of different genetic and posttranslational variants of human hemoglobin using ion mobility mass spectrometry.L39: Hydrogen deuterium exchange in protein molecules dissolved in water: Mechanism and kinetics of exchange.L40: Hydrogen/Deuterium exchange-based mass spectrometry (H/DX-MS).
Week 9:
L41: Application of H/DX-MS in assessing the conformational changes of proteins with an example of oxygenation of human hemoglobin.L42: Continued…..Application of H/DX-MS in assessing the conformational changes of proteins with an example of oxygenation of human hemoglobin.L43: In vivo study: Application H/DX-MS in the analysis of conformation change of human hemoglobin upon oxygenation within Red Blood Cells.L44: Application of H/DX-MS data in understanding the mechanism of polymerization of sickle hemoglobin (HbS) in sickle cell anemia.L45: Continued…..Application of H/DX-MS data in understanding the mechanism of polymerization of sickle hemoglobin (HbS) in sickle cell anemia.
Week 10:
L46: Application of H/DX-MS data in understanding the mechanism of inhibition of polymerization of HbS upon glutathionylation.L47: Continued…..Application of H/DX-MS data in understanding the mechanism of inhibition of polymerization of HbS upon glutathionylation.L48: Application of H/DX-MS in monitoring multiple conformation of hemoglobin variants in a mixed population.L49: Application of H/DX-MS in the identification and characterization of biosimilar compounds in the field of drug discovery.L50: Analysis of H/DX-MS data at the residue level of exchange using electron transfer dissociation.
Week 11:
L51: Analysis of a protein conformation using fast photochemical oxidation of proteins (FPOP).L52: Adsorption induced changes in the protein conformation on the surface of nano particles probed by HDX-MS.L53: Introduction of Imaging Mass Spectrometry (IMS) using MALDI and Desorption Electrospray Ionization (DESI) source.L54: Preanalytical steps in the processing a tissue section prior to imaging.L55: Application of MALDI-MS in imaging rat kidney tissues exposed to nano particles.
Week 12:

L56: Analysis of the recorded images of proteins in rat kidney tissues using tissue proteomics to identify and characterize the protein markers.L57: Application of MALDI-MS in imaging human prostate cancer tissues and cervical cancer tissues.L58: Continued…..Application of MALDI-MS in imaging human prostate cancer tissues and cervical cancer tissue.L59: Analysis of the recorded images of proteins in prostate cancer and cervical cancer tissues using tissue proteomics to identify and characterize the protein markers.L60: Application of DESI-MS in imaging human prostate cancer tissues.