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Classroom Contents
Breaking Through RT-qPCR Boundaries in Gene Expression Analysis
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- 1 Intro
- 2 Webinar Outline
- 3 Reverse Transcription Basics
- 4 Quantitative Reverse Transcription Polymerase Chain Reaction (RT-qPCR)
- 5 RT-qPCR Workflow
- 6 Reverse Transcription Components
- 7 Reverse Transcriptase Considerations
- 8 Reverse Transcriptase Attributes: RNase H Activity
- 9 Reverse Transcriptase Attributes: Thermostability
- 10 Reverse Transcriptase Attributes: Processivity
- 11 Reverse Transcriptase Attributes: Sensitivity
- 12 Reverse Transcriptase Attributes: Inhibitor Tolerance
- 13 How to Choose a Reverse Transcriptase?
- 14 Working with Challenging/Suboptimal RNA Samples
- 15 Detection of Low-input RNA and/or Low-abundance Genes
- 16 Working with Limited Sample Sources
- 17 Samples with High Structural Complexity
- 18 Working with (Archived) Tissue Samples: FFPE
- 19 Working with (Archived) Tissue Samples: Blood
- 20 Working with Plant Samples: Challenges
- 21 Working with Plant Samples: Inhibition
- 22 Difficult RNA Samples
- 23 Genomic DNA Contamination
- 24 gDNA Removal with DNases
- 25 gDNA Removal with ezDNase Enzyme
- 26 Conclusion
- 27 Thank You for Attending the Webinar
