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Explore advanced strategies for scaling mRNA production through fed-batch in vitro transcription (IVT) techniques in this 31-minute webinar. Discover how fed-batch IVT, which involves gradual reagent addition, can significantly enhance mRNA yield and improve reagent usage efficiency compared to traditional batch methods. Learn to utilize the Beckman Coulter BioLector XT Microbioreactor and Cytiva ReadyToProcess WAVEâ„¢ 25 rocking bioreactor for streamlined process development and scale-up operations. Examine how microfluidic feeding and real-time monitoring capabilities of the BioLector XT enable rapid optimization of feeding strategies and feed stock concentrations in sub-milliliter reactions. Understand how optimized fed-batch IVT conditions can achieve mRNA yields up to 15 g/L based on initial IVT volume while improving NTP incorporation efficiency and reducing DNA template usage. Investigate the linear correlation between pH and mRNA crude concentration as a non-invasive, real-time indicator for monitoring synthesis progress. See how process insights translate seamlessly from laboratory-scale optimization to the closed, GMP-ready ReadyToProcess WAVEâ„¢ 25 system, supporting IVT volumes ranging from 20 mL to 20 L for commercial manufacturing applications.
