ABOUT THE COURSE:The proposed course involves imparting knowledge of the instrumentation technique/spectroscopic methods. used to study and characterize new materials, analyzing toxicants in blood and serum samples, ensuring purty of the raw materials and final products. It can be used for both qualitative and quantitative analysis. The technique is mainly used in industries as well as research institutions and a must learn for students of Chemical Sciences, Physical Sciences, Biological Sciences as well as Engineering disciplines. Even numerous medical diagnostics are based on these instrumentation techniques.The course structure comprises various techniques that are the basis of modern-day research and medical diagnosis. This is highly job-oriented paper. The learners imbibe necessary skills for qualitative and quantitative analysis and would be offered jobs in industries, research labs and medical colleges as Analyst.INTENDED AUDIENCE:Pre-final and Final year students of UG (FYUP) and PG students, PhD scholars and industry personnel.PREREQUISITES: NAINDUSTRY SUPPORT:All Chemical and Pharmaceutical industries need experts in instrumental methods/spectroscopy so that they can effectively render their services Quality Control or R&D

Syllabus

Week 1:Principle of absorption and emission spectroscopy, Atomic absorption spectroscopy- Principle and working. Atomic emission spectroscopy- principle, instrumentation and working.

Week 2:Introduction to Inductively coupled plasma source (ICP), sample introduction, plasma appearance & spectra, analyte atomization. and ionization, direct current plasma sources and microwave plasma, plasma source spectrometers-slew-scan spectrometer, multi-channel spectrometers.

Week 3:Application of plasma, sources sample proportion, elements determined, line selection, calibration curves, interference & detection limits: comparison of ICP vs AAS.

Week 4:Theory of fluorescence and phosphorescence, factors affecting fluorescence and phosphorescence, Emission and excitation spectra.

Week 5:Relationship between concentration and fluorescence intensity, Jablonski diagram, delayed fluorescence, quenching.
Week 6:Instruments for measuring fluorescence and phosphorescence, Fluorometric determination of inorganic and organic species, problems.

Week 7:Introduction, classification of chromatographic methods, Elution chromatography on columns, Migration rates of solutes: distribution constants, retention time and retention factor, selectivity factor, zone broadening & column efficiency, variables that affect column performance, problems. normal phase & reversed phase packing chromatography.

Week 8:Method development in partition chromatography, column selection, mobile phase selection. Principle of HPLC, Structural types of column packing, column efficiency in liquid chromatography, Instrumentation and working.

Week 9:Types of detectors used (UV-visible, fluorescence detectors, Refractive index detector and Electrochemical detectors), number of theoretical plates, Applications problems.

Week 10:Principle, Instruments for gas liquid chromatography, mobile phase, sample injection, gas chromatographic columns, Liquid phase and column selection, classification of stationary phases.

Week 11:Detectors-flame ionization detector, thermal conductivity detector, Electron capture detector, thermionic detector and others.

Week 12:Interpretation of gas chromatographs, Temperature Programming in GC, Qualitive and quantitative analysis, problems.