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Learn a practical workflow combining ultracentrifugation with nano-flow cytometry for extracellular vesicle enumeration and integrity assessment in this 49-minute webinar. Explore how ultracentrifugation remains a widely used method for isolating extracellular vesicles despite ongoing debates around yield, integrity, and optimal protocols. Discover the integration of ultracentrifugation with nano-flow cytometry using the CytoFLEX nano system for comprehensive EV analysis. Examine how spin time impacts both pellet and supernatant yield through current literature review and in-house research data. Investigate a detailed case study exploring extracellular vesicle differences in obesity research. Understand calcein staining results that demonstrate vesicle integrity maintenance following ultracentrifugation processing. Gain practical insights into implementing and evaluating this combined analytical workflow within your own extracellular vesicle research projects. Access expert guidance on methodological challenges and biological roles of EVs in complex biological systems from a post-doctoral researcher specializing in extracellular vesicle measurement using novel fluorescent imaging approaches.
