Extracellular Vesicles Characterization and Isolation Through Nanoscale Flow Cytometry

Learn advanced techniques for characterizing and isolating extracellular vesicles (EVs) using cutting-edge nanoscale flow cytometry technology in this 57-minute webinar presented by Professor Claudia Maria Radu. Discover how extracellular vesicles serve as crucial mediators of intercellular communication, functioning as phospholipid bilayer-enclosed structures that cannot replicate but carry important molecular cargo between cells. Explore the significant challenges posed by EVs' nanoscale size and low refractive index when using conventional flow cytometers for detection and size determination. Understand why standard polystyrene beads complicate calibration processes due to their vastly different light-scattering properties compared to EVs. Master the capabilities of the CytoFLEX nano platform, a revolutionary instrument equipped with multiple-scatter laser technology that dramatically increases sensitivity for detecting small EVs. Gain expertise in combining scatter-based detection with fluorescence antibody labeling to simultaneously measure EV size, concentration, and surface marker expression. Develop skills in applying this more accurate and sensitive analytical approach to elucidate EVs' cellular origins, molecular cargo composition, and functional roles in both health and disease states.