Nanoparticle Isolation via Nano Scale Sorting - Small Footprint, Small Particles, and Big Ideas

Learn advanced techniques for isolating and sorting nanoparticles, specifically extracellular vesicles (EVs), through nano-scale flow cytometry and sorting methods in this comprehensive webinar. Explore the growing field of extracellular vesicle research and understand why these cellular components have become crucial subjects of study due to their ability to regulate gene expression and alter cellular functions across various cell types. Discover the technical challenges and recent hardware advancements in nano-flow cytometry that have enabled more precise analysis of EV populations, including efforts to standardize nano-flow data for improved research collaboration and comparison. Examine the complexities of nano-sorting techniques, which remain underutilized despite their potential, and review previous research demonstrating nanoscale vesicle sorting efficiencies of 25-45% using the MoFlo Astrios system. Investigate innovative approaches to enhance sorting efficiency through the CytoFLEX SRT platform for enriching EV populations and improving miRNA detection via molecular beacons. Gain insights from John Tigges, an experienced immunology specialist and flow cytometry expert with extensive background in stem cell biology research at Harvard Stem Cell Institute, who brings practical expertise in small particle detection and cutting-edge flow cytometry technologies to guide you through these advanced methodologies.