Coursera Spring Sale
40% Off Coursera Plus Annual!
Grab it
Explore the development and applications of reusable supports for protein and antibody purification in this 44-minute webinar presented by Chris Wojewodzki, an R&D Scientist III at Thermo Fisher Scientific with over 20 years of experience in manufacturing and R&D. Discover how high-capacity Ni-IMAC (Nickel Immobilized Metal Affinity Chromatography) and Protein A resins and magnetic beads can transform both manual and automated purification workflows. Learn about Ni-IMAC's effectiveness in purifying histidine-tagged proteins through the strong affinity between nickel ions and histidine residues, and understand how Protein A specializes in antibody purification by exploiting its high affinity for the Fc region of immunoglobulins. Examine the robust regeneration capabilities of both supports, which allow for multiple cycles of use without significant loss of binding capacity using standardized CIP (Clean in Place) protocols. Understand the versatility of Ni-IMAC for various sample types including cell lysates and culture supernatants, while exploring Protein A's predominant use for antibody purification from complex biological matrices such as serum, plasma, and cell culture media. Gain insights into how the combination of high specificity, efficient regeneration, and effective CIP protocols makes these supports indispensable tools in biotechnological and pharmaceutical applications.
