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Explore the transformative potential of reusable supports in protein and antibody purification through this 44-minute webinar that addresses critical challenges in biotechnology and pharmaceutical industries. Discover how advanced materials and innovative engineering solutions can replace traditional single-use methods that generate high costs and significant waste. Learn about the development and practical applications of reusable Thermo Fisher Scientific supports specifically designed for protein and antibody purifications, with comprehensive coverage of both manual and automated workflows utilizing high-capacity Ni-IMAC (Nickel Immobilized Metal Affinity Chromatography) and Protein A resins and magnetic beads. Understand the fundamental principles behind Ni-IMAC technology and its exceptional capability for purifying histidine-tagged proteins by leveraging the strong affinity between nickel ions and histidine residues. Examine Protein A supports and their specialization in antibody purification through exploitation of high affinity for the Fc region of immunoglobulins. Master the robust regeneration capabilities of both support types that enable multiple cycles of use without significant loss of binding capacity using standardized CIP (Clean in Place) protocols. Analyze the versatility of Ni-IMAC applications across various sample types including cell lysates and culture supernatants, while exploring Protein A's predominant use in antibody purification from complex biological matrices such as serum, plasma, and cell culture media. Gain insights into how the combination of high specificity, efficient regeneration, and effective CIP protocols makes these reusable supports indispensable tools for achieving enhanced efficiency, reduced environmental impact, and substantial cost savings in biotechnological and pharmaceutical applications.
