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Learn advanced protein engineering techniques to address charge-driven clearance issues in monoclonal antibody development through this 19-minute webinar presented by Dr. Md Tariqul Haque Tuhin from Amgen. Explore the mechanisms behind target-independent clearance of immunoglobulins, focusing on non-specific endocytosis and neonatal Fc receptor-mediated cellular recycling processes. Examine a clinical case study of an anti-IL-4Rα monoclonal antibody that exhibited high clearance rates due to four problematic arginine residues in its variable domain. Discover how strategic point mutations can reduce exposed positive charge and improve antibody pharmacokinetics. Compare the effectiveness of variable domain mutations versus YTE Fc mutations in decreasing clearance liabilities, and understand how combining both approaches optimizes FcRn recycling efficiency. Gain insights into establishing in vitro ADME assay workflows for biologics development and learn about cell-based methods for studying therapeutic protein disposition. Access PACE credits through Labroots registration and webinar completion, with credits available until February 2027.
