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Computational Enhanced Multicontrast Imaging of Organoids and In Vivo Mouse Brain

Center for Physical Genomics and Engineering via YouTube

Overview

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Explore cutting-edge computational imaging techniques that revolutionize biological visualization through this 59-minute conference talk by Dr. Xi Chen from Northwestern University. Discover how artificial intelligence and quantitative phase imaging (QPI) are transforming the study of living systems, from cellular structures to complex tissues. Learn about artificial confocal microscopy, a groundbreaking label-free 3D imaging approach that achieves confocal-like resolution and molecular specificity in spheroids and organoids without traditional labeling methods. Examine AI-driven methodologies for evaluating embryo health through integrated multimodal imaging data analysis. Delve into the innovative integration of QPI with multiphoton microscopy for deep tissue imaging applications. Understand the development and applications of Scattering-Enabled Epi Quantitative Phase Imaging (SEEQPI), a novel technique combining long-wavelength illumination, confocal sectioning, and phase-shifting interferometry to achieve unprecedented deep tissue penetration in mouse brain imaging. Gain insights into how this laser-scanning, common-path architecture enables stable, high-sensitivity phase measurements at low excitation powers, significantly reducing photodamage while facilitating longitudinal studies in live animal models. Perfect for researchers, biomedical engineers, and scientists interested in advanced optical imaging, computational biology, and next-generation microscopy techniques for studying complex biological systems.

Syllabus

Xi (Dawn) Chen - Computational Enhanced Multicontrast Imaging of Organoids and In Vivo Mouse Brain

Taught by

Center for Physical Genomics and Engineering

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