Coursera Flash Sale
40% Off Coursera Plus for 3 Months!
Grab it
Learn the comprehensive chemistry and workflows for operating the Illumina Genome Analyzer through this intensive boot camp covering sample preparation, cluster generation, sequencing processes, and data management. Master the fundamental techniques including DNA shearing, size selection, SPRI purification, and sample tracking protocols essential for successful genomic sequencing. Explore cluster generation chemistry, QPCR optimization, quality control measures using SYBR, and troubleshooting common failure modes encountered during the process. Understand sequencing chemistry principles, first base incorporation protocols, and workflow optimization strategies used in high-throughput genomic analysis. Gain practical experience through hands-on laboratory demonstrations showing sample preparation techniques, cluster generation procedures, and sequencing operations. Develop proficiency in data pipeline workflows, file management systems, and troubleshooting methodologies for analyzing sequencing output and resolving technical issues that may arise during genome analyzer operations.
