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YouTube

Illumina Sequencing Overview - Library Prep to Data Analysis

Ambry Genetics via YouTube

Overview

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Explore an in-depth webinar on Illumina sequencing, covering the entire process from library preparation to data analysis. Gain insights into the Illumina Sequencing Workflow for Next Generation Sequencing, including library preparation constructs, cluster generation, and sequencing by synthesis. Learn about the biochemical processes automatically performed by Illumina sequencing devices and compare different chemistry versions and instrument engineering developments. Presented by Scott Westenberger, PhD, Staff Clinical Field Applications Scientist at Illumina, this 52-minute session provides a comprehensive overview applicable to various assays across different sequencing instruments. Delve into topics such as flow cell architecture, bridge amplification, dye chemistry, paired-end sequencing, and analysis overview. Moderated by Tara Namey, MS, LCGC, Senior Genomic Science Liaison at Ambry Genetics, this webinar offers valuable knowledge for professionals in molecular biology, genetics research, and clinical oncology.

Syllabus

Intro
Session Objectives
Illumina Sequencing Workflow
Library Prep is Critical for Successful Sequencing
What is a Cluster?
What is a flow cell?
Flow cell Architecture Random vs Pattemed
Patterned flow cells Complete control of pitch & feature size
Hybridize Fragment & Extend
Denature Double-Stranded DNA
Single-Stranded DNA
Bridge Amplification
Denature Double-Stranded Bridge
Linearization
Reverse Strand Cleavage
Read 1 Primer Hybridization
Patterned Flow Cell and ExAmp Technology
Illumina Sequencing Systems
Dye Chemistry 4-channel chemistry
2-Channel SBS Chemistry
Dye Chemistry 2-channel chemistry
Sequencing by synthesis with CMOS detection 1-channel chemistry
Illumina Chemistry Comparison
Paired-End Sequencing
Single Index Reads
Dual Index Reads
Analysis Overview

Taught by

Ambry Genetics

Reviews

5.0 rating, based on 5 Class Central reviews

Start your review of Illumina Sequencing Overview - Library Prep to Data Analysis

  • Vanessa Junco Ruisánchez
    This course provides a comprehensive and easy-to-follow overview of Illumina sequencing, from library preparation to data analysis. It effectively explains each step of the process, making it an excellent resource for both beginners and those looking to refresh their knowledge. The video content is clear, and the visuals help illustrate complex concepts, such as sequencing technology and bioinformatics workflows. I found the explanations of data quality control and analysis to be particularly valuable. Highly recommend for anyone interested in sequencing technologies and bioinformatics.
  • Profile image for Pamela Melo
    Pamela Melo
    This course offers a comprehensive introduction to the Illumina sequencing workflow, covering every critical stage from library preparation to data analysis. It's an excellent resource for beginners in genomics, lab technicians, or researchers looking to get a solid grounding in next-generation sequencing (NGS) using Illumina platforms.
  • Athira K
    It was really good, all information is useful I'm really new to this , this video really helped me .
  • Elliott Rodriguez-Lopez
    This is a well-detailed presentation. It is highly recommended if you are trying to learn the basic details of Next-generation sequencing, how the Illumina machine works, and their major differences.
  • Profile image for Mihir Trivedi
    Mihir Trivedi
    That was very informative course for beginners and who want to know what next generation sequencing is.

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