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Overview
Syllabus
Intro
Session Objectives
Illumina Sequencing Workflow
Library Prep is Critical for Successful Sequencing
What is a Cluster?
What is a flow cell?
Flow cell Architecture Random vs Pattemed
Patterned flow cells Complete control of pitch & feature size
Hybridize Fragment & Extend
Denature Double-Stranded DNA
Single-Stranded DNA
Bridge Amplification
Denature Double-Stranded Bridge
Linearization
Reverse Strand Cleavage
Read 1 Primer Hybridization
Patterned Flow Cell and ExAmp Technology
Illumina Sequencing Systems
Dye Chemistry 4-channel chemistry
2-Channel SBS Chemistry
Dye Chemistry 2-channel chemistry
Sequencing by synthesis with CMOS detection 1-channel chemistry
Illumina Chemistry Comparison
Paired-End Sequencing
Single Index Reads
Dual Index Reads
Analysis Overview
Taught by
Ambry Genetics
Reviews
5.0 rating, based on 5 Class Central reviews
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This course provides a comprehensive and easy-to-follow overview of Illumina sequencing, from library preparation to data analysis. It effectively explains each step of the process, making it an excellent resource for both beginners and those looking to refresh their knowledge. The video content is clear, and the visuals help illustrate complex concepts, such as sequencing technology and bioinformatics workflows. I found the explanations of data quality control and analysis to be particularly valuable. Highly recommend for anyone interested in sequencing technologies and bioinformatics.
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This course offers a comprehensive introduction to the Illumina sequencing workflow, covering every critical stage from library preparation to data analysis. It's an excellent resource for beginners in genomics, lab technicians, or researchers looking to get a solid grounding in next-generation sequencing (NGS) using Illumina platforms.
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It was really good, all information is useful I'm really new to this , this video really helped me .
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This is a well-detailed presentation. It is highly recommended if you are trying to learn the basic details of Next-generation sequencing, how the Illumina machine works, and their major differences.
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That was very informative course for beginners and who want to know what next generation sequencing is.