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Learn about groundbreaking spatial omics research techniques in this 57-minute webinar presented by experts Kendra Ritchie, Joseph Melko, and Dr. Sara Bodbin. Explore the innovative combination of HCR™ Pro RNA-FISH and in situ proximity ligation assay (isPLA) technologies for simultaneous detection of RNA expression and protein interactions. Discover how these cutting-edge methods can be automated using the BOND RX research stainer to study tissue phenotypes from a functional perspective. Master the technical aspects of the HCR™ platform for RNA-ISH assays and understand how isPLA technology can be integrated for comprehensive sample analysis. Through a practical demonstration focusing on the PD1/PD-L1 inhibitory pathway, gain insights into detecting both transcript expression and protein interactions in the same sample. Understand the significance of combining transcriptomics and proteomics data for more complete biological insights, particularly given that transcript abundance doesn't always correlate with protein expression levels. Learn how the BOND RX automated research stainer's flexibility enables seamless integration of multiple advanced technologies to accelerate research discoveries.
Syllabus
Assessing differential expression of protein interactions and RNA by combining isPLA and...
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