Simultaneous Gene Expression Analysis and Fusion Identification by Pairing the New KAPA RNA EvoPrep Kit with KAPA HyperExome V2 Probes

Attend this 21-minute webinar presented by Dr. Amy Elias from Roche Sequencing and Life Science to explore a novel RNA sequencing workflow that addresses key limitations in transcriptome profiling. Learn how the new KAPA RNA EvoPrep Kit paired with KAPA HyperExome V2 Probes enables simultaneous gene expression analysis and fusion detection by enriching protein-coding genes from both high-quality and formalin-fixed paraffin-embedded (FFPE) samples. Discover how this innovative approach overcomes traditional RNA-seq challenges including high input mass requirements, inefficient fusion detection, and wasted sequencing reads on non-coding transcripts. Examine the workflow's consistency across different input types and masses, and explore its capability to detect clinically relevant fusion transcripts and exon-skipping events that are present at low but biologically significant levels in tumor samples. Gain insights into key advancements in RNA library preparation methodology that provides comprehensive transcriptome data while maintaining efficient fusion detection capabilities. The presentation demonstrates practical applications using real sample data and offers solutions for laboratories seeking to optimize their RNA sequencing workflows for both research and clinical diagnostics applications.