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Explore critical pre-analytical factors that significantly impact RNA integrity and downstream analytical quality in cancer transcriptomics research through this 26-minute webinar. Learn how transcriptomics enables comprehensive mapping of gene expression profiles and identification of fusion genes implicated in tumor development, while understanding that robust transcriptomic data depends heavily on high-quality RNA extraction processes. Discover how pre-analytical variables such as blood collection tube selection and extraction chemistries directly affect the reliability of cancer transcriptomics results, with particular emphasis on the challenges posed by formalin-fixed paraffin-embedded (FFPE) tissue samples commonly used in clinical research and archival studies. Examine data demonstrating how variations in FFPE tissue extraction chemistry can lead to inconsistent fusion gene detection, potentially causing researchers to overlook critical genetic information essential for cancer biomarker discovery and therapeutic targeting. Gain insights from workflow analyses of FFPE-derived RNA that highlight optimization strategies for improving sensitivity and specificity in fusion gene identification. Master best practices for sample extraction and handling from both blood-derived and FFPE tissues to enhance the translational value and reliability of cancer transcriptomic research, reinforcing the foundational importance of RNA quality in advancing cancer research outcomes.
