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Single Molecule Light Field Microscopy - A Revolution in 3D Detection

Labroots via YouTube

Overview

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Join a comprehensive webinar exploring the groundbreaking developments in Light Field Microscopy (LFM) and its revolutionary application to single molecule detection. Learn how this innovative technique, first introduced in 2006, captures complete 3D volumes in a single snapshot with digital refocusing and deconvolution capabilities. Discover how Prof. Steve Lee's research group at the University of Cambridge is advancing LFM for single molecule localization microscopy, achieving fast, high-density volumetric imaging using just a seven-microlens array. Gain insights from expert speakers including Prof. Steve Lee and Dr. Sam Daly from Cambridge University, and Jeremy Graham from Cairn Research, who details the crucial microlens array design. Explore the technical requirements for successful LFM implementation, including high-sensitivity CMOS cameras and custom software solutions, with specific focus on the Kinetix CMOS camera and PySMLFM open source Python script developed by Teledyne Photometrics. Master the fundamentals of LFM technology, understand its transformative impact on single molecule localization, and examine the essential tools and techniques driving the future of 3D microscopy.

Syllabus

Single Molecule Light Field Microscopy, a Revolution in 3D Detection

Taught by

Labroots

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