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Explore advanced microscopy techniques for studying cell surface receptor interactions in this 39-minute webinar presented by postdoctoral researcher Katie Sharrocks from the Francis Crick Institute. Learn how single-molecule super-resolution microscopy enables researchers to investigate the dimerization and oligomerization of membrane protein receptors, including G-protein coupled receptors (GPCRs) and receptor tyrosine kinases (RTKs), which are central to cellular signaling pathways and represent targets for approximately 34% of FDA-approved drugs. Discover how super-resolution microscopy techniques surpass traditional diffraction-limited light microscopy to accurately determine individual membrane receptor locations and their oligomeric states on cell surfaces. Examine specific applications through detailed discussion of D2 dopamine GPCR research, where super-resolution microscopy revealed differences in receptor oligomerization between mutant receptors. Gain insights into the critical role of receptor dimerization in cellular function, from essential dimerization requirements for some receptors to mechanisms for increasing signaling diversity in others. Understand the practical applications of these techniques in studying Epidermal Growth Factor Receptor di/oligomerization in Non-Small Cell Lung Cancer cell lines and their potential implications for future patient treatments.
