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Molecular Biology Advanced

Shomu's Biology via YouTube

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Overview

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Dive deep into advanced molecular biology concepts through a comprehensive 4.5-hour tutorial covering protein production, DNA recombination, mutation types, and DNA repair mechanisms. Explore topics such as Holliday junction resolution, CSSR recombination, Cre-lox system, and landmark experiments like photo reactivation and Luria-Delbrück. Examine various mutation types including chromosomal, genetic, and DNA mutations, as well as their effects on gene expression. Gain in-depth knowledge of DNA repair processes, including double-strand break repair models, methyltransferase enzymes, transcription-coupled repair, and non-homologous end joining. Enhance your understanding of advanced molecular biology principles essential for research and academic pursuits.

Syllabus

Protein production.
DNA recombination basic.
Holliday junction resolution.
Types of CSSR recombination.
Serine recombinase.
Tyrosine recombinase.
Cre lox recombination system.
Photo reactivation experiment.
Luria delbruck experiment.
Spontaneous mutation.
Mutation changes the gene expression.
Chromosomal mutation.
Genetic mutation, DNA mutation (point mutation).
Transition and transversions.
Frameshift mutation.
Nonsense mutations.
DNA repair overview.
DNA repair mechanism.
DNA repair mechanisms.
Double strand break repair model.
Methyltransferase enzyme.
Transcription coupled repair.
Translesion DNA synthesis.
Synthesis dependent strand annealing.
Single strand annealing.
Double strand break repair by homologus recombination.
Non homologous end joining.
Non homologous double stranded break repair.

Taught by

Shomu"s Biology

Reviews

5.0 rating, based on 2 Class Central reviews

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  • Ali Usman
    Advanced molecular biology and gene mutation is a very important and fascinating field of study. It helps us understand how life works at the molecular level and how changes in DNA can affect living organisms. This subject explains the structure and function of genes, DNA replication, transcription, translation, and the regulation of gene expression.
    One of the most interesting parts of this topic is gene mutation. Gene mutations are changes in the DNA sequence, and they can happen naturally or due to environmental factors such as radiation or chemicals. These mutations can be harmless, beneficial, or harmful. Studying gene mutations is essential because they are linked to genetic diseases, cancer, and evolution.
  • Anuoluwapo Olamide Adigun
    Great course.
    In a short while, I have been able to learn a lot. I will be able to use this knowledge gained to progress in my career and in my studies. Although the videos are blur, I hope they get better.

    Thank you.
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