Seed Train and Bioprocess Strategies for Suspension Cell Culture-Based Adeno-Associated Virus Production

Learn about seed train and bioprocess strategies for suspension cell culture-based adeno-associated virus (AAV) production in this 54-minute webinar presented by Ines Hartmann and Dr. Stefan Schlößer. Explore how cell culture-produced AAV vectors have become essential tools for gene and protein delivery in vaccine production and gene therapy development. Discover the advantages of using single-use bioreactors with precise control over temperature, pH, medium agitation, and gassing composition for scalable AAV capsid production. Examine the establishment of an efficient production platform based on Expi293F suspension cell culture and learn how shake flask seed trains using CO2 incubator shakers can prepare sufficient cell numbers for bioreactor inoculation. Compare the advantages and disadvantages of adherent versus suspension cell culture for different applications, understand various seed train strategies for preparing adequate inoculum, and gain insights into process development for AAV production in single-use bioreactors. The webinar addresses critical aspects of process scale-up options and accurate process parameter control necessary for maintaining reliable and reproducible AAV production workflows.