Overview
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Learn how to accelerate viral vector production for gene and cell therapy applications through this 22-minute webinar presented by Dr. Shan Gao from FUJIFILM Irvine Scientific. Discover the challenges of current viral vector production workflows that rely on basal media in batch-mode, which often require lengthy and costly optimization of HEK293 cell culture conditions. Explore the development and performance of BalanCD HEK293 Viral Feed, a chemically defined feed medium specifically designed to boost viral vector yields in both fed-batch suspension and adherent cell cultures. Examine research results demonstrating that adding the feed medium 24 hours post-transfection increases adeno-associated virus (AAV) titer by 3-fold to 10-fold and improves lentivirus (LV) genome and functional titer by 3-fold. Understand how this simplified and effective workflow can achieve increased viral titer production in high cell density HEK293 cultures, addressing the growing demand for cost-effective cell and gene therapy manufacturing. Gain insights from Dr. Gao's expertise in protein engineering, molecular diagnostics, viral vectors for gene delivery, and cell culture media development for gene therapy and vaccine applications.
Syllabus
BalanCD HEK293 Viral Feed: Accelerating AAV and LV Production for Gene and Cell Therapy
Taught by
Labroots