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Explore advanced RNA guide engineering techniques for programmable CRISPR-Cas9 control in DNA data storage and computational systems through this 15-minute conference presentation. Discover how CRISPR-Cas9's precision in targeted DNA editing creates new possibilities for digital data storage by enabling direct rewriting and retrieval of information encoded in DNA molecules. Learn about the integration of Smart RNA guides that conditionally activate Cas9 in response to specific RNA/DNA triggers, allowing for rapid, real-time assessment of guide enzyme variants and accelerated optimization cycles for CRISPR-based systems. Understand how conditional control mechanisms enable Boolean logic operations including AND, NOT, and XOR functions, creating a programmable layer that refines Cas9 activity based on complex cellular or environmental inputs. Examine how this approach functions as a natural cellular processor where DNA serves as high-density memory while enzymes and RNA molecules operate as logic circuits manipulating stored information. Gain insights into the paradigm that bridges biological and computational systems, offering blueprints for next-generation in-memory computing devices that seamlessly integrate data storage and processing at the molecular level. The presentation covers the implementation of dynamic, reconfigurable DNA storage systems where information can be updated, recorded, or erased on demand, establishing programmable frameworks for managing DNA-based data with precision and flexibility.
