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This webinar, presented by Michael Collis, Application Specialist at Spherotech, Inc., explores quality control considerations for flow cytometry and imaging particles. Learn about essential definitions, flow cytometer anatomy, and common manufactured and data acquisition ranges. Discover alignment protocols, calibration tools, and cross-calibration techniques necessary for reliable flow cytometry results. The presentation covers how to set up standard operating procedures for measurable parameters, define and implement calibration processes, understand Molecules of Equivalent Fluorescence (MEF) units, and apply MEF values to unknown populations for statistically significant research determinations. By the end of this 66-minute session, gain the knowledge to perform daily instrument QC with Levy Jennings Graphs for CV and MFI tracking, conduct proper instrument calibration to attain calculated MEF, Rsq, and Residual Values, and successfully cross-calibrate MEF values for unknown populations. The webinar concludes with insights on low-end sensitivity and background measurements using Q and B values.
