Optimizing Laboratory-Developed LC-MS/MS Tests - Proper Use of Internal Standards and Insights from Two Troubleshooting Case Studies

Learn to optimize laboratory-developed LC-MS/MS tests through proper internal standard selection and implementation in this 32-minute webinar presented by Dr. Meng Wang, a Research & Development Scientist at Vancouver Coastal Health specializing in mass spectrometry method development and clinical laboratory quality improvement. Discover how stable isotope-labeled internal standards normalize variations throughout the analytical process, including sample preparation, injection, chromatography, and mass spectrometry ionization, while exploring the key criteria for selecting appropriate internal standards for small molecule analytes. Examine two detailed troubleshooting case studies from quality improvement projects: resolving non-linear response issues in urine 5-Hydroxyindoleacetic acid (5-HIAA) testing and addressing high imprecision problems in Sirolimus testing at low concentrations. Understand how switching to more suitable internal standards can dramatically improve accuracy, linearity, and precision in clinical assays, while learning to identify and avoid common pitfalls that can lead to biased clinical results despite following general guidelines. Gain practical insights into mass spectrometry method optimization, laboratory workflow automation, and quality control measures essential for reliable clinical testing. Earn PACE credits by registering as a Labroots member and completing the webinar requirements before the November 13, 2026 expiration date.