Super-Resolution Microscopy: SIM, STED, STORM and PALM Techniques - Lecture 9

Explore advanced microscopy techniques in this 27-minute lecture focusing on strategies for resolution enhancement in super-resolution microscopy. Learn about three major super-resolution techniques: Structured Illumination Microscopy (SIM), which uses patterned illumination to extract high-resolution information; Stimulated Emission Depletion Microscopy (STED), which achieves nanoscale resolution through selective fluorophore deactivation; and the related techniques of Stochastic Optical Reconstruction Microscopy (STORM) and Photoactivated Localization Microscopy (PALM), which enable single-molecule imaging through precise control of fluorescent molecules.