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Explore cutting-edge advancements in super-resolution fluorescence microscopy and single-cell transcriptome imaging in this illuminating conference talk. Delve into the development of stochastic optical reconstruction microscopy (STORM), a groundbreaking technique that overcomes the diffraction limit of light microscopy using photo-switchable fluorescent probes. Discover how STORM enables multicolor and three-dimensional imaging of living cells with nanometer-scale resolution, leading to novel sub-cellular structure discoveries. Learn about the innovative multiplexed error-robust fluorescent in situ hybridization (MERFISH) method, which allows for imaging and quantification of numerous RNA species in single cells. Gain insights into how these advanced imaging techniques facilitate the delineation of gene regulatory networks and in situ identification of cell types, pushing the boundaries of our understanding of cellular biology at the nanoscale.
