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This lecture by Dr. Kristy Stengel explores how rapid transcription factor degradation techniques reveal critical mechanisms of gene regulation. Learn about innovative CRISPR-mediated genome editing approaches that introduce degron tags into endogenous transcription factor loci, allowing for rapid degradation following PROTAC treatment. Discover how this chemical-genetic method collapses the timeframe for assaying transcriptional changes from days to hours, overcoming limitations of traditional slow genetic and knockdown strategies. The presentation details how Dr. Stengel's team defined the mechanism of action of the RUNX1 transcription factor on gene regulation and enhancer function, explaining its role in myeloid development and how its disruption contributes to pathologic gene expression in myeloid leukemia. Dr. Stengel, an assistant professor at Albert Einstein College of Medicine, brings expertise from her Ph.D. in Cell and Molecular Biology and postdoctoral work at Vanderbilt University to this 59-minute talk sponsored by the Center for Physical Genomics and Engineering, the Cancer and Physical Sciences Program, and NIH Grants.
