How to Manipulate Gene Expression Data from NCBI GEO in R Using Dplyr - Bioinformatics for Beginners

Learn to manipulate gene expression data from NCBI GEO using R and dplyr in this hands-on tutorial for bioinformatics beginners. Set up RStudio, read RNA-Seq data, retrieve metadata with GEOquery, and perform data manipulation using essential dplyr functions. Master techniques such as selecting, renaming, and mutating columns, reshaping data with gather(), joining datasets, filtering, grouping, summarizing, and arranging results. Follow along with provided code and resources to enhance your skills in analyzing genomic data efficiently.

Syllabus

Intro
Requirements
Set up RStudio for Analysis
Read data in R
Get metadata using GEOquery package
dplyr: select, rename, mutate
Reshape data: gather
Join data: left_join
dplyr: filter, group_by, summarize, arrange