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Learn advanced gene expression analysis techniques in this comprehensive tutorial that guides through filtering differentially expressed genes and performing functional annotations using bioinformatics tools. Master the process of working with .csv files from GEO2R, filtering DEGs based on Log Fold Change and P-values, and conducting Gene Ontology annotations across biological processes, molecular functions, and cellular components. Explore pathway enrichment analysis using Enrichr for web-based annotations and create powerful visualizations of GO and pathway networks through ClueGO and CluePedia in Cytoscape. Gain practical experience with essential bioinformatics tools including GEO2R, Enrichr, Cytoscape, and associated plugins while developing the skills to confidently interpret gene expression data and extract meaningful biological insights.
