rRNA Sequencing Analysis and Visualization

This course demonstrates downstream analysis of 16S rRNA microbiome data, covering OTU clustering, phylotype analysis, alpha and beta diversity, PCA, AMOVA, and phylogenetic-tree visualization. It also shows how to inspect and visualize results using R, Galaxy, Krona, and MEGAN6.

Syllabus

Introduction
Inputs required
Analyses Included in this video
Goals for this video
Looking at the input files format
OTU identification and clustering
Phylotype Analysis
Alpha and Beta Diversity
PCA output in R
AMOVA between treatments
ggtree for tree plotting in R
Customization of ggtree
Galaxy + Krona
MEGAN6
Closing summary