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NPTEL

Fundamentals of Bioprocess Engineering

NPTEL via Swayam

Overview

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ABOUT THE COURSE:This course emphasizes the basic understanding of the bioprocess engineering. It starts with the fundamentals of the bioprocess engineering, its importance and role in the industrial biotechnology. It also covers the introductory part of the bioreactor, types, modes of operation and the various products of economic importance. The course discusses the microbial and enzyme kinetics. It highlights the importance of metabolic stoichiometry in media formulation. It also includes the mass transfer principles, downstream processing techniques and thus it will be helpful for the students and scholars of interdisciplinary domains. The course content was prepared in such a way that it will be applicable for UG, PG students and PhD scholars.INTENDED AUDIENCE: Under graduate and post graduate students, professional in the discipline of Biotechnology and Chemical EngineeringPREREQUISITES: Bachelor Degree in any Science/Engineering disciplineINDUSTRY SUPPORT: Industries dealing with Bioprocess and fermentation technology like Biocon; Wockhardt; India Glycol Limited; Jubilant; Lupin

Syllabus

Week 1: Introduction to Bioprocess engineering; Metabolic stoichiometry
  • Overview of the fermentation process
  • Strain isolation and the process flow (Upstream and Downstream)
  • Metabolic pathways
  • Cell nutrients
  • Industrially important products
Week 2:Metabolic stoichiometry and energetics
  • Material and mass balance
  • Material balance for substrate intake and product formation
  • Bioprocess thermodynamics
  • Theoretical Yield
Week 3:Enzyme kinetics
  • Basics of enzyme catalysis
  • Michaelis Menton Kinetics
  • Types of enzyme kinetics (Single and bi-substrate)
  • Enzyme inhibition
Week 4:Applied enzyme catalysis
  • Types of enzyme immobilization
  • External and internal mass transfer
  • Minimizing mass transfer effects
  • Applications of enzymes
Week 5:Microbial kinetics
  • Microbial growth kinetics
  • Monod Equation
  • Transient growth kinetics
  • Types of metabolites (primary and secondary)
Week 6:Kinetics of substrate utilization, product formation and biomass production in cell cultures
  • Various types of structured kinetic models
  • Kinetics of balanced growth
  • Kinetics of substrate uptake
  • Product formation kinetics
Week 7:Design and analysis of biological reactors
  • Introduction to bioreactors
  • Design and components of the reactor
  • Various modes of Operation
  • Types (CSTR, PFR, Packed Bed, Membrane and Photo Bioreactor)
  • Bioreactors for animal and plant cells
Week 8:Gas-Liquid mass transfer in bioprocess systems
  • Mass transfer theories
  • Diffusion and the relevant theories
  • Role of aeration and agitation
  • Oxygen transfer from bubbles to the cells
  • Measurement of oxygen mass transfer coefficient
Week 9:Heat transfer in bioprocess systems; Scale-up
  • Heat Transfer in Bioprocessing
  • Cell death kinetics
  • Batch and Continuous Sterilization
  • Heat transfer equipment
  • Bioprocess scale up criteria
Week 10:Bioprocess instrumentation and control
  • Sensors for bioreactors
  • Measurement of process parameters
  • Process control Systems
  • Process dynamics
  • Controllers
Week 11:Product recovery
  • Importance of Downstream Processing
  • Various techniques used in product recovery
  • Cell separation and disruption
  • Centrifugation, filtration, sedimentation
  • Extraction and Chromatography
Week 12:Product recovery; Bioprocess economics.
  • Adsorption
  • Membrane separation
  • Drying
  • Introduction to the Bioprocess economics
  • Economic analysis of the fermentation process

Taught by

Prof. Lalit M. Pandey

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