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0:00 Intro
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Classroom Contents
NGS Data 101 - FASTQ Files, Library Preparation, and Lane Multiplexing
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- 1 0:00 Intro
- 2 00:34 Why so many FASTQ files for 1 sample?
- 3 01:34 Segment 1: Library Preparation, Barcodes and Multiplexing
- 4 02:54 Why would same sample have different library prep?
- 5 04:17 Segment 2: Flowcell
- 6 04:49 Why run multiple samples on the same flowcell?
- 7 05:51 Why run the same sample across multiple lanes in a flowcell?
- 8 06:42 Single-Lane vs Multi-Lane Coverage
- 9 08:10 Looking at data from a real-life example
- 10 09:27 Understanding library preparation and sequencing for Genome in a Bottle GIAB dataset
- 11 13:43 Understanding folder structure and data organization
- 12 17:35 Illustrating the library preparation workflow
- 13 19:24 Segment 3: How to handle these multiple FASTQs?
