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Explore the critical issue of genetic instability in human pluripotent stem cells through this 31-minute webinar presented by Dr. Ivana Barbaric from the University of Sheffield. Delve into the molecular mechanisms underlying genome integrity maintenance in hPSCs and understand how disruption of these processes leads to undesired genetic changes, particularly recurrent aneuploidies. Learn about the non-random genetic alterations that provide selective growth advantages to variant cells and their concerning associations with oncogenesis, raising important safety questions for regenerative medicine applications. Discover how aneuploid hPSCs can dominate mosaic cultures through competitive interactions that eliminate wild-type cells, similar to cell competition behaviors observed in other developmental models. Examine the functional effects of aneuploidy on hPSC behavior in vitro and understand how these genetic changes can compromise both basic research reliability and clinical safety. Gain insights into strategies for minimizing genetic variant occurrence in hPSC cultures intended for research and therapeutic use, with implications extending to assisted conception and potential cancer therapies targeting aneuploid cells. Master the identification of common genetic changes in hPSCs, evaluate the challenges these changes present for regenerative medicine, and discuss how understanding mutation and selection processes can inform approaches to reduce genetic variant appearance in stem cell cultures.
