Validation and Standardization of DNA Extraction and Library Construction Methods for Metagenomics-Based Human Fecal Microbiome Measurements

Join a 33-minute webinar led by Dr. Dieter Tourlousse, senior scientist at the National Institute of Advanced Industrial Science and Technology (AIST), exploring the standardization of metagenomics-based human fecal microbiome measurements. Learn about systematic protocol comparisons for DNA extraction and library construction using novel mock communities, developed through the Japan Microbiome Consortium (JMBC). Discover the results of industry-based interlaboratory studies and the MOSAIC Standards Challenge that assessed protocol reproducibility and transferability. Gain insights into established standard operating procedures (SOPs) and best-practice guidelines designed to enhance the accuracy and consistency of next-generation DNA sequencing-based metagenomic analyses across laboratories. Understand how these standardized methods, including the QIAseq FX DNA Library Kit for library construction, address the challenges of protocol-dependent bias and measurement variability in microbiome profiling.