Real Time Tracking of Root Hair Nucleus Morphodynamics Using a Microfluidic Approach
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Overview
Syllabus
Intro
Nuclear mechanics and dynamics in plant development
Nuclear shape changes in plant development
Mechanical stress reaching the nucleus?
Importance of the plant nucleo-cytoplasmic interphase
Change in nuclear shape starts beyond a threshold
Morphodynamics and mechanics of nucleus are reversible
Nucleus as a rheostat in plant development
Root hair (RH): a perfect model to study nuclear dynamics
Coverslip based microfluidic device (CMD)
Arabidopsis RH analysis: a microfluidic approach
Experimental procedure
Nuclear dynamics during tip growth
Nuclear dynamics: changes in nuclear shape during tips
Nuclear dynamics during RH development
First conclusions
Further exploration of microfluidic approaches
Nuclear mechano-transduction in plant development
Acknowledgements
Taught by
Society for Experimental Biology