Synthetically Primed Adaptation of Bacterial Metabolism to Renewable Sugar Substrates

Explore the cutting-edge field of bacterial metabolic engineering in this 31-minute webinar presented by Dr. Pavel Dvořák from Masaryk University. Discover how synthetic biology approaches can prime bacterial adaptation to renewable sugar substrates, focusing on the biotechnological workhorse Pseudomonas putida and its potential for valorizing lignocellulosic waste materials. Learn about the challenges microorganisms face when utilizing renewable sugars like xylose and cellobiose, and understand why many biotechnologically relevant bacteria prefer glucose over pentoses or lack efficient aromatic compound metabolism. Examine the comprehensive research methodology combining rational genetic interventions with adaptive laboratory evolution to establish efficient substrate utilization in cell factories. Delve into multi-omics analyses, enzyme assays, and reverse engineering techniques used to elucidate and improve semi-synthetic xylose metabolism and glucose-cellobiose co-metabolism in P. putida. Understand how knowledge-directed metabolic engineering can prime further bacterial adaptation during laboratory evolution, and explore the descriptive and predictive capabilities of upgraded genome-scale metabolic models constrained by kinetic and proteomic data. Gain insights into the holistic understanding of adaptation processes in engineered microorganisms and their applications for biotechnological valorization of renewable feedstocks. The presentation demonstrates practical approaches for exploiting semi-synthetic metabolism in bacterial systems and provides fundamental knowledge for understanding bacterial adaptation to novel substrates, making it valuable for researchers in metabolic engineering, synthetic biology, and sustainable biotechnology.