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Explore the advanced capabilities of spectral flow cytometry through this 59-minute webinar presented by Dr. Tamar Tak and Dr. Fanuel Messaggio from the Flow Cytometry Core Facility at Leiden University Medical Center. Discover how the CytoFLEX mosaic Spectral Detection Module transforms existing CytoFLEX instruments by adding full-spectrum cytometry capabilities, demonstrated through comprehensive beta-testing results from a CytoFLEX mosaic-88 coupled to a 6-laser CytoFLEX LX flow cytometer. Learn to discriminate between fluorochromes with highly similar excitation and emission spectra, including challenging combinations like APC and Alexa Fluor 647, and understand how to effectively incorporate these into complex multi-parameter panels. Master techniques for extracting multiple autofluorescent signatures from heterogeneous samples to enhance data quality and resolution, while examining the successful transfer of a sophisticated 30-color panel designed for analyzing murine metabolic tissues (OMIP-104) to the CytoFLEX mosaic platform. Gain insights into leveraging the six side scatter detectors for label-free identification of distinct cell populations, providing practical knowledge for implementing spectral cytometry in your research workflow.
