Overview
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Learn essential quality control principles for real-time PCR gene expression experiments in this 53-minute webinar presented by Dr. John Pfeifer, an expert with over three decades of PCR experience. Navigate the complexities of qPCR gene expression analysis by implementing rigorous quality control measures to ensure data integrity and reproducibility in eukaryotic systems. Discover how to validate assay specificity and optimize transcript coverage while identifying and mitigating potential inhibition factors that could compromise your results. Master the implementation of effective controls and learn strategies for managing biogroup variation that can impact experimental outcomes. Explore key considerations for designing robust gene expression experiments with built-in quality control steps from the planning phase through execution. Gain practical insights into ensuring precise, reproducible results that meet the highest standards of scientific rigor. The presentation covers critical aspects of experimental design, troubleshooting common issues, and establishing protocols that maintain consistency across different experimental conditions. Earn PACE continuing education credits upon completion by following the provided registration and completion process through the Labroots platform.
Syllabus
Quality Control of Real-Time PCR Gene Expression Experiments
Taught by
Labroots