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This webinar, presented by Dr. Dan Beacham, Senior Staff Scientist at Thermo Fisher Scientific, explores fluorescence imaging techniques for analyzing cell surface target internalization in cancer and immunology research. Learn practical considerations for assay development, including platform selection and newly developed probes for studying target internalization, trafficking, phagocytosis, and endocytic mechanisms of action. Discover how to implement concomitant or serial readouts for apoptosis, cell health, and stress as companion assays for Antibody-Drug Conjugate (ADC) labeling, delivery, and lysosomal degradation. Master best practices in live-cell imaging assay development, techniques for leveraging endosomal pH to quantify internalization and trafficking, and the effective use of pathway and endpoint indicators for cell health and stress assessment.
